Cellular distribution of type I and type II receptors for transforming growth factor-beta.
1986; Elsevier BV; Volume: 261; Issue: 21 Linguagem: Inglês
10.1016/s0021-9258(18)67611-2
ISSN1083-351X
AutoresSela Cheifetz, B Like, Joan Massagué,
Tópico(s)Estrogen and related hormone effects
ResumoAffinity labeling of target cells for transforming growth factor+ (TGFB) by cross-linking with lZsI- TGFb via disuccinimidyl suberate or by the photoreactive analogue 4-a~idobenzoyl-'~~I-TGFB has revealed the presence of multiple TGFB receptor forms.Two distinct types of TGFb receptors can be distinguished based on structural analysis of the '261-TGFB-labeled species by peptide mapping.Type I TGFB receptors include the 280-kilodalton labeled receptor form previously found to be the subunit of a disulfide-linked TGFB receptor complex.(Massague, J. (1985) J. Biol.Chern.260, 7059-7066), as well as a 65-kDa labeled receptor form present in all cell lines examined, and a 130-140-kDa labeled receptor form detected only in 3T3-Ll cells.The 280-kDa form is the major TGFB receptor species in most cell lines examined, but is apparently absent in rat skeletal muscle myoblasts.Type I TGFB receptors bind TGFB with an apparent Kd of 50-500 pM.Type 11 TGFB receptors include an 85-kDa labeled receptor form present in all mammalian cells examined and a 110-kDa labeled receptor form present in chick embryo fibroblasts.Type I1 TGFB receptors bind TGFB with an apparent Kd of about 50 PM.Except for the 280-kDa type I TGFB receptor form, none of the TGFB receptor forms described here is found as part of a disulfide-linked receptor complex.All the TGFB receptor forms described here behave as intrinsic membrane proteins exposed on the surface of intact cells.Transforming growth factor-@ (TGFP'), a 25-kDa polypeptide hormone from blood platelets and other cell types, is a potent regulator of cell development (reviewed in Refs. 1 and 2).TGFP induces anchorage-independent growth of normal fibroblasts in semisolid medium (3), but it also inhibits other forms of cell growth (4, 5) and controls programs of cell differentiation including adipogenesis (6) and myogenesis.'Many cell types whose development is regulated by TGFP
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