Quantitative analysis of amoxicillin, its major metabolites and ampicillin in eggs by liquid chromatography combined with electrospray ionization tandem mass spectrometry
2015; Elsevier BV; Volume: 192; Linguagem: Inglês
10.1016/j.foodchem.2015.07.028
ISSN1873-7072
AutoresLirui Sun, Longfei Jia, Xing Xie, Kaizhou Xie, Jianfeng Wang, Jianyu Liu, Lulu Cui, Genxi Zhang, Guojun Dai, Jinyu Wang,
Tópico(s)Pharmaceutical and Antibiotic Environmental Impacts
ResumoIn this present study, we developed a simple, rapid and specific method for the quantitative analysis of the contents of amoxicillin (AMO), AMO metabolites and ampicillin (AMP) in eggs. This method uses a simple liquid–liquid extraction with acetonitrile followed by liquid chromatography–tandem mass spectrometry (LC–MS/MS). The optimized method has been validated according to requirements defined by the European Union and Food and Drug Administration. Extraction recoveries of the target compounds from the egg at 5, 10 and 25 μg/kg were all higher than 80%, with relative standard deviations not exceeding 10.00%. The limits of quantification in eggs were below the maximum residue limits (MRLs). The decision limits (CCα) ranged between 11.1 and 11.5 μg/kg, while detection capabilities (CCβ) from 12.1 to 13.0 μg/kg. These values were very close to the corresponding MRLs. Finally, the new approach was successfully verified for the quantitative determination of these analytes in 40 commercial eggs from local supermarkets.
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