Rapid Purification of 70 S RNA from Media of Cells Producing RNA Tumor Viruses
1976; American Society for Microbiology; Volume: 17; Issue: 1 Linguagem: Inglês
10.1128/jvi.17.1.287-290.1976
ISSN1098-5514
AutoresR. Guy Smith, L A Donehower, Robert C. Gallo, David Gillespie,
Tópico(s)Bacteriophages and microbial interactions
ResumoMedia from cells producing RNA tumor viruses, when treated with sodium dodecyl sulfate and polyvinyl sulfate, yield 70S RNA as the major species binding oligo(dT)-cellulose. The procedure described for purifying 70S RNA requires no special equipment and is suitable for rapidly processing large quantities of media or for purifying RNA from commercially avialable virus, with a 5- to 10-fold higher yield than was obtained using existing methods.
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