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Effect of dietary supplementation with Bacillus subtilis on the growth, performance, immune response and antioxidant activities of the shrimp (Litopenaeus vannamei)

2010; Wiley; Volume: 41; Issue: 11 Linguagem: Inglês

10.1111/j.1365-2109.2010.02554.x

1365-2109

Wen-Ying Shen, Linglin Fu, Weifen Li, Yao-Rong Zhu,

Aquatic life and conservation

Aquaculture ResearchVolume 41, Issue 11 p. 1691-1698 Effect of dietary supplementation with Bacillus subtilis on the growth, performance, immune response and antioxidant activities of the shrimp (Litopenaeus vannamei) Wen-Ying Shen, Wen-Ying Shen Department of Biology, Life Science College of Shaoxing University, Shaoxing, China The Key Laboratory of Molecular Animal Nutrition, Animal Science College, Zhejiang University, Ministry of Education, Hangzhou, ChinaSearch for more papers by this authorLing-Lin Fu, Ling-Lin Fu Department of Food Quality and Safety, Zhejiang Gongshang University, Hangzhou, ChinaSearch for more papers by this authorWei-Fen Li, Wei-Fen Li The Key Laboratory of Molecular Animal Nutrition, Animal Science College, Zhejiang University, Ministry of Education, Hangzhou, ChinaSearch for more papers by this authorYao-Rong Zhu, Yao-Rong Zhu Department of Biology, Life Science College of Shaoxing University, Shaoxing, ChinaSearch for more papers by this author Wen-Ying Shen, Wen-Ying Shen Department of Biology, Life Science College of Shaoxing University, Shaoxing, China The Key Laboratory of Molecular Animal Nutrition, Animal Science College, Zhejiang University, Ministry of Education, Hangzhou, ChinaSearch for more papers by this authorLing-Lin Fu, Ling-Lin Fu Department of Food Quality and Safety, Zhejiang Gongshang University, Hangzhou, ChinaSearch for more papers by this authorWei-Fen Li, Wei-Fen Li The Key Laboratory of Molecular Animal Nutrition, Animal Science College, Zhejiang University, Ministry of Education, Hangzhou, ChinaSearch for more papers by this authorYao-Rong Zhu, Yao-Rong Zhu Department of Biology, Life Science College of Shaoxing University, Shaoxing, ChinaSearch for more papers by this author First published: 01 July 2010 https://doi.org/10.1111/j.1365-2109.2010.02554.xCitations: 118 Correspondence: W-F Li, The Key Laboratory of Molecular Animal Nutrition, Animal Science College, Zhejiang University (Hua Jia Chi Campus), Ministry of Education, Hangzhou 310029, China. E-mail: wfli@zju.edu.cn Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract The objective of this study was to evaluate the effect of dietary supplementation of a probiotic bacterium, Bacillus subtilis, on the growth, immune response and antioxidant activities of shrimp (Litopenaeus vannamei). Shrimps with an average initial weight of 2.11±0.17 g were randomly assigned to four groups with three replicates. The control group was fed a basal diet, and three treated groups were fed diets supplemented with B. subtilis at doses of 1 × 104, 5 × 104 and 10 × 104 colony-forming unit (CFU) g−1 feed respectively. After 40 days of culture, 10 shrimps from each replicate were taken randomly for the determination of immune response and oxidization resistance indices. The results showed that the shrimps fed with B. subtilis at a dose of 1 × 104, 5 × 104 CFU g−1 feed showed significantly better growth than that of the control diet. The phenoloxidase activities showed a tendency to increase with an increased dose of B. subtilis in diets but there was no significant difference among the three treated groups. In addition, phenoloxidase activities were found to be significantly higher (P<0.05) in the groups treated with B. subtilis than that of the control group. Shrimps treated with 5 × 104 CFU g−1 feed probiotic bacterium showed the highest lysozyme activity and it was significantly higher (P<0.05) than the other groups. However, there was no significant difference in acid phosphatase and alkaline phosphatase activity across all the groups. The total antioxidant capacity, superoxide dismutase and glutathione peroxidase activities in the probiotic-treated groups were significantly increased (P<0.05) as compared with the control groups. Both maleic dialdehyde concentration and superoxide anion activities in the probiotic-treated groups were significantly lower (P<0.05) than those of the control. The probiotic did not affect the nitric oxide synthase and the catalase activity in any of the control and treated groups. These results indicated that the probiotic B. subtilis could significantly promote the growth rate of shrimp by increasing the immune function and antioxidant capacity. The most effective dose of B. subtilis in the diet was 5 × 104 CFU g−1 feed. Citing Literature Volume41, Issue11October 2010Pages 1691-1698 RelatedInformation