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The Complete Primary Structure of the Phosphatidylcholine-Transfer Protein from Bovine Liver. Isolation and Characterization of the Cyanogen Bromide Peptides

1981; Wiley; Volume: 114; Issue: 2 Linguagem: Inglês

10.1111/j.1432-1033.1981.tb05158.x

1432-1033

Robert Akeroyd, Peter Moonen, Jan Westerman, W. C. Puyk, K.W.A. Wirtz,

Lipid Membrane Structure and Behavior

The complete primary structure of the phosphatidylcholine transfer protein from bovine liver consists of: Ac‐Met‐Asp‐Pro‐Gly‐Ala‐Gly‐Ala‐Phe‐Ser‐Glu 10 ‐Glu‐Gln‐Phe‐Arg‐Glu‐Ala‐Cys‐Ala‐Glu‐Leu 20 ‐Gln‐Arg‐Pro‐Ala‐Leu‐Ser‐Gly‐Ala‐Ala‐Trp 30 ‐Glu‐Leu‐Leu‐Val‐Glu‐Thr‐Gln‐Gly‐Ile‐Ser 40 ‐Val‐Tyr‐Arg‐Leu‐Leu‐Asp‐Gln‐Gln‐Thr‐Gly 50 ‐Leu‐Tyr‐Ala‐Tyr‐Lys‐Val‐Phe‐Gly‐Val‐Leu 60 ‐Glu ‐Asp‐Cys‐Leu‐Pro‐Asp‐Leu‐Leu‐Ala‐Asp 70 ‐Val‐Tyr‐Met‐Asp‐Leu‐Ala‐Tyr‐Arg‐Lys‐Gln 80 ‐Trp‐Asp‐Gln‐Tyr‐Val‐Lys‐Glu‐Leu‐Tyr‐Glu 90 ‐Lys‐Glu‐Cys‐Ser‐Gly‐Glu‐Thr‐Val‐Val‐Tyr 100 ‐Trp‐Gin‐Val‐Lys‐Tyr‐Pro‐Phe‐Pro‐Met‐Ser 110 ‐Asn‐Arg‐Asp‐Tyr‐Val‐Tyr‐Val‐Arg‐Gln‐Arg 130 ‐Gln‐Glu‐Leu‐Asp‐Phe‐Glu‐Gly‐Gln‐Lys‐Val 130 ‐His‐Val‐Ile‐Leu‐Ala‐Gln‐Ser‐Thr‐Ser‐Glu 140 ‐ Pro‐Gln‐Phe‐Pro‐Glu‐Lys‐Ser‐Gly‐Val‐Ile 150 ‐Arg‐Val‐Lys‐His‐Tyr‐Lys‐Gln‐Arg‐Leu‐Ala 160 ‐Ile‐Gin‐Ser‐Asp‐Gly‐Lys‐Arg‐Gly‐Ser‐Lys 170 ‐Val‐Phe‐Met‐Tyr‐Tyr‐Phe‐Asp‐Asn‐Pro‐Gly 180 ‐Gly‐Gln‐Ile‐Pro‐Ser‐Trp‐Val‐Ile‐Asn‐Trp 190 ‐Ala‐Ala‐Lys‐Asn‐Gly‐Val‐Pro‐Asn‐Phe‐Leu 200 ‐Lys‐Asp‐Met‐Val‐Lys‐Ala‐Cys‐Gln‐Asn‐Tyr 210 ‐Lys‐Lys‐Thr. Elucidation of the sequence was based on digestion of the protein by cyanogen bromide, trypsin, protease from Staphylococcus aureus , protease from mouse submaxillaris gland and clostripain, and the manual and automated Edman degradation of the peptides. The sequence of the N‐terminal half (residues 1–122) has been previously published [Moonen, P., Akeroyd, R., Westerman, J., Puyk, W. C., Smils, P., and Wirtz, K. W. A. (1980) Eur. J. Biochem. 106 , 279–290]. The protein contains two disulfide bridges at Cys 17 ‐Cys 63 and Cys 93 ‐Cys= 207 . It has a calculated molecular weight of 24681 and a molar absorbance at 280 nm of 60365 M −1 cm −1 .