Complete assignment of the 1H nuclear magnetic resonance spectrum of French bean plastocyanin
1988; Elsevier BV; Volume: 202; Issue: 3 Linguagem: Inglês
10.1016/0022-2836(88)90290-2
ISSN1089-8638
AutoresWalter Chazin, Mark Ranee, Peter E. Wright,
Tópico(s)Molecular spectroscopy and chirality
ResumoThe identification of the spin systems that comprise the 1H nuclear magnetic resonance spectrum of French bean Cu(I) plastocyanin (Mr 10,600) has been made using an approach that integrates a wide range of two-dimensional nuclear magnetic resonance experiments. A very large percentage of these assignments has been obtained in spectra acquired from 1H2O solution using a backbone amide-based strategy. The spin systems of 91 of the 99 residues have been assigned to the appropriate amino acid, thereby providing an ample basis for obtaining sequence-specific assignments, as described in the accompanying paper.
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