Portal de Periódicos da CAPES

Changes induced by oxygen in rat liver proteins identified by high‐resolution two‐dimensional gel electrophoresis

2000; Wiley; Volume: 267; Issue: 17 Linguagem: Inglês

10.1046/j.1432-1327.2000.01627.x

1432-1033

Cristina Miralles, Àlvar Agustí, Christiane Aubry, Jean-Charles Sánchez, Claude Walzer, Denis F. Hochstrasser, Xavier Busquets,

Amino Acid Enzymes and Metabolism

Molecular oxygen (O 2 ) regulates the expression of a variety of genes. Several of the proteins that respond to changes in oxygen concentration have been identified in a variety of cell lines. We extend these previous studies by analyzing the effect of oxygen on the entire protein expression profile of an intact organ using high‐resolution two‐dimensional gel electrophoresis. To this end, we used an isolated, in vitro perfused organ preparation to produce two groups of rat livers perfused with high (95% O 2 , 5% CO 2 ) or low (95% N 2 , 5% CO 2 ) oxygen concentrations. Using two‐dimensional gel electrophoresis we compared the protein expression profiles of both groups of livers. Computer analysis of the files obtained after laser densitometry of the two‐dimensional gels revealed two spots that were strongly up‐regulated in high PO 2 perfused livers compared with low PO 2 perfused livers. These spots were analyzed by peptide mass fingerprinting analysis. These spots were identified as arginase 1 (liver‐type arginase; EC 3.5.3.1) and mitochondrial enoyl‐CoA hydratase 1 (EC 4.2.1.17). The possible role of these proteins in its new context of oxygen availability is discussed.