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Effects of Liposome-Encapsulated Hemoglobin on Learning Ability in Tokai High-Avoider Rat After Total Brain Ischemia and Reperfusion

2014; Wiley; Volume: 38; Issue: 8 Linguagem: Inglês

10.1111/aor.12352

1525-1594

Akira T. Kawaguchi, Hitoshi Endo, Hiroyuki Aikawa, Mariko Yamano, Yo Kawaguchi, Munetaka Haida, Tetsu Watanabe,

Biochemical effects in animals

Artificial OrgansVolume 38, Issue 8 p. 667-674 Main Text Article Effects of Liposome-Encapsulated Hemoglobin on Learning Ability in Tokai High-Avoider Rat After Total Brain Ischemia and Reperfusion Akira T. Kawaguchi, Corresponding Author Akira T. Kawaguchi Department of Cell Transplantation and Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, JapanAddress correspondence and reprint requests to Dr. Akira T. Kawaguchi, Tokai University School of Medicine, Cell Transplantation and Regenerative Medicine, Isehara, Kanagawa 259-1193, Japan. E-mail: akira@is.icc.u-tokai.ac.jpSearch for more papers by this authorHitoshi Endo, Hitoshi Endo Basic Clinical Science and Public Health, Tokai University School of Medicine, Isehara, Kanagawa, JapanSearch for more papers by this authorHiroyuki Aikawa, Hiroyuki Aikawa Basic Clinical Science and Public Health, Tokai University School of Medicine, Isehara, Kanagawa, JapanSearch for more papers by this authorMariko Yamano, Mariko Yamano Integrated Rehabilitation, Osaka Prefecture University, Habikino, Osaka, JapanSearch for more papers by this authorYo Kawaguchi, Yo Kawaguchi Department of Cell Transplantation and Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, JapanSearch for more papers by this authorMunetaka Haida, Munetaka Haida Tokai University Junior College of Nursing and Medical Technology, Hiratsuka, Kanagawa, JapanSearch for more papers by this authorTetsu Watanabe, Tetsu Watanabe Basic Clinical Science and Public Health, Tokai University School of Medicine, Isehara, Kanagawa, JapanSearch for more papers by this author Akira T. Kawaguchi, Corresponding Author Akira T. Kawaguchi Department of Cell Transplantation and Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, JapanAddress correspondence and reprint requests to Dr. Akira T. Kawaguchi, Tokai University School of Medicine, Cell Transplantation and Regenerative Medicine, Isehara, Kanagawa 259-1193, Japan. E-mail: akira@is.icc.u-tokai.ac.jpSearch for more papers by this authorHitoshi Endo, Hitoshi Endo Basic Clinical Science and Public Health, Tokai University School of Medicine, Isehara, Kanagawa, JapanSearch for more papers by this authorHiroyuki Aikawa, Hiroyuki Aikawa Basic Clinical Science and Public Health, Tokai University School of Medicine, Isehara, Kanagawa, JapanSearch for more papers by this authorMariko Yamano, Mariko Yamano Integrated Rehabilitation, Osaka Prefecture University, Habikino, Osaka, JapanSearch for more papers by this authorYo Kawaguchi, Yo Kawaguchi Department of Cell Transplantation and Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, JapanSearch for more papers by this authorMunetaka Haida, Munetaka Haida Tokai University Junior College of Nursing and Medical Technology, Hiratsuka, Kanagawa, JapanSearch for more papers by this authorTetsu Watanabe, Tetsu Watanabe Basic Clinical Science and Public Health, Tokai University School of Medicine, Isehara, Kanagawa, JapanSearch for more papers by this author First published: 28 July 2014 https://doi.org/10.1111/aor.12352Citations: 11 Presented in part at the 4th International Symposium on Artificial Oxygen Carriers, held September 28, 2013, in Yokohama, Japan. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Liposome-encapsulated hemoglobin with low O2-affinity (l-LEH) was shown to be protective in focal brain ischemia and reperfusion (I/R) in rats and primates. We tested l-LEH in the transient whole brain ischemia in the Tokai high-avoider rat (THA), which has been selected, mated, and bred over 77 generations for a high and consistent learning ability determined by the Sidman avoidance test (SAT). Young/naïve (before SAT) and adult/parent (after SAT) THA rats underwent acute and complete four-vessel occlusion in the chest for 3 or 5 min, administration of 2 mL/kg of l-LEH, saline, or homologous washed red blood cells (RBCs), reperfusion, and resuscitation. One week later, all rats underwent SAT, open-field behavioral observation, Morris water maze tests, and morphological study. Whereas young/naïve rats treated with l-LEH retained a rapid and consistent learning curve as in nonischemic controls, THA rats treated with RBCs or saline had retarded learning response on SAT as well as reduced cellularity in the amygdala. Adult/parent rats with established memory on SAT maintained perfect achievement even after I/R. In contrast, l-LEH-treated rats showed no better performance on Morris water maze (function) or cellularity of the CA1 sector of the hippocampus (morphology) compared with the rats treated with RBCs. Although task performance on SAT and Morris water maze appeared antithetical, morphological observations corresponded to the respective functions, suggesting that l-LEH was protective only for the amygdala on SAT tasks but not for the CA1 sector of the hippocampus on spatial orientation as in our previous studies on focal brain I/R, where the cortex was preserved better than basal ganglia. Citing Literature Volume38, Issue8August 2014Pages 667-674 RelatedInformation