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Studies on passage of the hemadsorbing factor of murine tumors to normal cell cultures.

1967; National Institutes of Health; Volume: 27; Issue: 11 Linguagem: Inglês

Loren J. Humphrey,

Toxin Mechanisms and Immunotoxins

Summary The hemadsorbing factor of methylcholanthrene-induced sarcoma (MC-Sa) was studied in passage experiments. Frozen and thawed suspensions of MC-Sa excised from mice or grown in cell culture were inoculated into tubes containing primary mouse embryo tissue culture (METC). After incubation for three to five days the contents of these tubes were frozen and thawed and passed at a dilution of 1:10 into a new primary METC. Positive hemadsorption tests were obtained after five passages of the MC-Sa-inoculated METC. Passage controls of METC and uninoculated METC controls failed to hemadsorb. Surprisingly, METC inoculated with frozen and thawed suspension of murine kidney also hemadsorbed after five passages, while METC inoculated with a similar preparation of melanoma failed to hemadsorb. In addition, METC inoculated with this MC-Sa preparation and tested for hemadsorption demonstrated a growth curve similar to that seen in viral studies. These studies suggest that a virus-like agent is associated with the hemadsorption by MC-Sa. Furthermore, it would appear that this agent is present in normal murine kidney and unmasked during passage to METC. The relationship of this hemadsorbing agent to carcinogenesis is not defined by these experiments. Consequently, studies are underway to evaluate the interrelationship of carcinogens and this hemadsorbing factor.