Calcium and magnesium content of rat liver and Morris Hepatoma 5123 tc.
1967; National Institutes of Health; Volume: 27; Issue: 6 Linguagem: Inglês
Autores Tópico(s)
Liver Disease Diagnosis and Treatment
ResumoCalcium and magnesium contents were determined by atomic absorption spectrophotometry in acid digests of normal rat livers, and of livers and tumors from rats bearing the “minimum-deviation” morris Hepatoma 5123 tc implanted subcutaneously. Water and nitrogen contents were also determined. Measurements were made on whole tissue, as well as on parenchyma. The hepatoma contained 79% water versus 71% in normal liver; the proportion of nitrogen in the solid matter of the tumor was higher than normal, indicating a decrease in non-nitrogenous components, presumably glycogen and lipids. On either wet or dry weight basis, the hepatoma contained at least 100% more calcium and 25% more magnesium than normal liver. The calcium content appeared to increase progressively with increasing age of the tumor. These results would not be anticipated on the basis of Coman's hypothesis that decreased adhesiveness of cancer cells is the result of decreased Ca++-binding ability of the cell membranes. However, they are not necessarily in conffict with it. The observed calcium accumulation in the tumor may be intracellular, perhaps by formation of calcium-citrate complexes. Host liver contained slightly, but significantly, more calcium than normal liver. This is taken to indicate an indirect metabolic effect of the tumor upon the host liver.
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