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Establishment and Culture of Human Skin Fibroblasts

2005; Wiley; Volume: 71; Issue: 1 Linguagem: Inglês

10.1002/0471142727.mb2803s71

1934-3639

Jacob Villegas, Michael J. McPhaul,

Protein Degradation and Inhibitors

Abstract The establishment of skin fibroblast strains provides a vehicle by which biochemical and genetic studies may be applied. In addition to providing genetic material to study the basis of disease, fibroblast cell lines established from skin biopsies may provide the investigator with a model in which to study specific disease states or normal physiology. This unit provides methods for the biopsy, establishment, and culture maintenance of skin fibroblasts to be used in further scientific study.