Retention and resolution in density-programmed super- critical fluid chromatography
1986; Elsevier BV; Volume: 369; Linguagem: Inglês
10.1016/s0021-9673(00)90097-9
ISSN1873-3778
AutoresKarl Heinz Linnemann, Anneliese Wilsch, Gerhard Schneider,
Tópico(s)Protein purification and stability
ResumoExperimental retention time data have been obtained from density-programmed supercritical fluid chromatography of dodecyl phenyl ether, using Perisorb RP-8 and Spherisorb ODS-2 as stationary phases and carbon dioxide as a mobile phase. They are compared with values calculated from a theoretical treatment of linear sample velocities during a density programme proposed recently. The numerical integration method presented earlier is confirmed for different density gradients. The effect of a density programme on the peak width, σl, was also investigated; under the conditions of the experiments, σl does not change much with density.
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