Isolation of L Forms from Group A Streptococci Exposed to Bacitracin
1965; American Society for Microbiology; Volume: 89; Issue: 6 Linguagem: Inglês
10.1128/jb.89.6.1581-1585.1965
ISSN1098-5530
AutoresJ Rotta, Walter W. Karakawa, Richard M. Krause,
Tópico(s)Natural product bioactivities and synthesis
ResumoRotta, Jiri (Washington University School of Medicine, St. Louis, Mo.), Walter W. Karakawa, and Richard M. Krause . Isolation of L forms from group A streptococci exposed to bacitracin. J. Bacteriol. 89: 1581–1585. 1965.—L forms were obtained from group A streptococci by exposure to bacitracin on gradient plates, although novobiocin was ineffective in this respect. Subcultures of these L forms had morphological and bacteriological properties similar to those obtained with penicillin. M protein was detected in L-form colony smears by immunofluorescent staining with type-specific conjugate. The L forms were not stained with group-specific conjugate. Parallel precipitin tests performed with extracts from a heavy growth of L forms on agar confirmed these findings. Thus, the L forms obtained with bacitracin continue to produce M protein but are devoid of the group-specific carbohydrate which is a major component of cell-wall structure.
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