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Two-dimensional gel analysis of swine histocompatibility antigens.

1983; American Association of Immunologists; Volume: 131; Issue: 6 Linguagem: Inglês

10.4049/jimmunol.131.6.2939

1550-6606

Barbara A. Osborne, Joan K. Lunney, Larry R. Pennington, D H Sachs, Stuart Rudikoff,

Animal Virus Infections Studies

Miniature swine MHC antigens from three inbred herds were examined by two-dimensional gel electrophoresis. These antigens were found to constitute a series of complex glycoproteins displaying haplotype-specific patterns that allowed the distinction of both class I and class II molecules among the three haplotypes. Selected outbred pig antisera reacted with a subset of class I antigens, suggesting the presence of at least two distinct molecular species among these antigens. Similarly, alloantisera reacting with mouse Ia antigens and a monoclonal anti-human DR were shown to immunoprecipitate a subset of class II molecules. Examination of the cells from two recombinant haplotypes demonstrated that both independent recombinational events took place between the class I and class II genes.