On-Column Approach in the HPLC-UV Analysis of Non-chromophoric Compounds Using Azelaic Acid as a Model
2012; Volume: 5; Issue: 3 Linguagem: Inglês
ISSN
2707-6253
AutoresMuhammed Alzweiri, Yusuf Al‐Hiari, Talal Aburjaı, Osama Abdel-Aldaem,
Tópico(s)Molecular Sensors and Ion Detection
ResumoThe manuscript introduces a detection solution of compounds lacking chromophoric properties (e.g. azelaic acid) by implementing a HPLC-UV analysis with on-column derivatisation of the analyte. Azelaic acid was used to test the feasibility of the method at λmax = 265 nm. Its chromatographic analysis shows linear (R = 0.999), precise (RSD < 2.0%) and accurate (97.0 - 103.5 %) behavior. Furthermore, the method was found selective for azelaic acid in a prepared cream which contains other ingredients such as triethanolamine, vaseline and stearic acid. The limit of detection (LOD) and limit of quantification (LOQ) of azelaic acid were 9 and 30 µg/ml, respectively.
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