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Nuclear and mitochondrial phylogenies provide evidence for four species of Eurasian badgers (Carnivora)

2010; Wiley; Volume: 39; Issue: 5 Linguagem: Inglês

10.1111/j.1463-6409.2010.00436.x

1463-6409

Irene Del Cerro, Josep Marmi, Aïnhoa Ferrando, П. В. Чащин, Pierre Taberlet, Montse Bosch,

Evolution and Paleontology Studies

Zoologica ScriptaVolume 39, Issue 5 p. 415-425 Nuclear and mitochondrial phylogenies provide evidence for four species of Eurasian badgers (Carnivora) Irene Del Cerro, Irene Del CerroSearch for more papers by this authorJosep Marmi, Josep MarmiSearch for more papers by this authorAïnhoa Ferrando, Aïnhoa FerrandoSearch for more papers by this authorPavel Chashchin, Pavel ChashchinSearch for more papers by this authorPierre Taberlet, Pierre TaberletSearch for more papers by this authorMontse Bosch, Montse BoschSearch for more papers by this author Irene Del Cerro, Irene Del CerroSearch for more papers by this authorJosep Marmi, Josep MarmiSearch for more papers by this authorAïnhoa Ferrando, Aïnhoa FerrandoSearch for more papers by this authorPavel Chashchin, Pavel ChashchinSearch for more papers by this authorPierre Taberlet, Pierre TaberletSearch for more papers by this authorMontse Bosch, Montse BoschSearch for more papers by this author First published: 16 August 2010 https://doi.org/10.1111/j.1463-6409.2010.00436.xCitations: 34 Corresponding author: Irene del Cerro, Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, Edifici V. Campus de la UAB, 08193-Bellaterra (Cerdanyola del Vallès), Barcelona, Spain. E-mail: irene.delcerro@uab.catAïnhoa Ferrando, Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, Edifici V. Campus de la UAB, 08193-Bellaterra (Cerdanyola del Vallès), Barcelona, Spain. E-mail: ainhoa.ferrando@uab.catJosep Marmi, Institut Català de Paleontologia, Mòdul ICP, Campus de la UAB, 08193 – Bellaterra (Cerdanyola del Vallès), Barcelona, Spain. E-mail: josep.marmi@icp.catPavel V. Chashchin, Ilmensky State Reserve, Russian Academy of Sciences. Ural Branch, 456317 – Miass, Russia. E-mail: olga@ilmeny.ac.ruPierre Taberlet, Laboratoire d'Ecologie Alpine, CNRS UMR 5553, Université Joseph Fourier, B.P. 53, F-38041 Grenoble Cedex 9, France. E-mail: pierre.taberlet@ujf-grenoble.frMontserrat Bosch, Departament de Genètica de la Conservació, Institut de Recerca i Tecnologia Agroalimentàries, Ctra. de Cabrils km2, 08348-Cabrils, Barcelona, Spain. E-mail: montse.bosch.gallego@gmail.com Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Del Cerro, I., Marmi, J., Ferrando, A., Chashchin, P., Taberlet, P. & Bosch, M. (2010). Nuclear and mitochondrial phylogenies provide evidence for four species of Eurasian badgers (Carnivora). —Zoologica Scripta, 39, 415–425. The Eurasian badgers (Meles spp.) have a fairly widespread distribution in the Palearctic region and their great morphological variability throughout the vast geographic area has nourished an intense debate about the classification of this taxon. Therefore, the aim of this study was to clarify controversies in Eurasian badger taxonomy by means of a new molecular phylogeny. One-hundred and seventeen individuals of Eurasian badgers from 18 countries throughout Eurasia were sequenced for up to 3257 bp of nuclear DNA over six loci (ACTC, BGN, CFTR, CHRNA1, TS and TTR) and 512 bp of the mitochondrial DNA control region. Statistical and phylogenetic analyses for combined nDNA, mtDNA and the total-evidence data clearly showed a strong genetic differentiation in four well-supported clades, three of which corresponded to allopatric badger species previously defined according to morphological data: Meles meles Linnaeus, 1758 in Europe; Meles leucurus Hodgson, 1847 in the continental part of Asia, except the south-west part; and M. anakuma Temminck, 1844 in Japan. Up to now, the fourth clade, made up of individuals from south-west Asia, had been considered as a subspecies. Supported by several pieces of morphological evidence, the new phylogeny revealed that it is necessary to revise the current taxonomic classification of Meles spp. and suggested that the badgers from south-west Asia should be recognised as a separate species, being renamed M. canescens Blanford, 1875. Citing Literature Supporting Information Appendix S1 Description of locus symbol, name, location and region amplified, primer sequences, PCR product size and PCR conditions used in this study. Appendix S2 Description of gene acronym and name, new allele-specific primer sequences and PCR conditions used in this study. Appendix S3 Geographic distribution and frequencies of Eurasian badger haplotype sequences by loci used in this study. GenBank accession numbers are also indicated. The number of haplotype sequences is 2n for nuclear loci and n for mitochondrial DNA. Appendix S4 Pairwise differences among species within Mustela and Martes genera and among the four Eurasian badger species calculated using the 'Minimatrix' data set (upper diagonal). Values within species are in the middle diagonal and Fst values among Meles species are in the lower diagonal. Accession numbers are shown in the Table below. Appendix S5 Models of evolution and parameters estimated using Modeltest for all data sets. Please note: Wiley-Blackwell are not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article. Filename Description ZSC_436_sm_appendixS1.doc52 KB Supporting info item ZSC_436_sm_appendixS2.doc38.5 KB Supporting info item ZSC_436_sm_AppendixS3.doc506.5 KB Supporting info item ZSC_436_sm_appendixS4.doc60.5 KB Supporting info item ZSC_436_sm_appendixS5.doc48 KB Supporting info item Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume39, Issue5September 2010Pages 415-425 RelatedInformation