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Costimulatory action of glycoinositolphospholipids from Trypanosoma cruzi: increased interleukin 2 secretion and induction of nuclear translocation of the nuclear factor of activated T cells 1
1999; Wiley; Volume: 13; Issue: 12 Linguagem: Inglês
10.1096/fasebj.13.12.1627
ISSN1530-6860
AutoresMaria Bellio, Ana Carolina Oliveira, Cláudia Mermelstein, Márcia Alves Marques Capella, João P. B. Viola, Jean‐Pierre Levraud, George A. DosReis, José O. Previato, Lúcia Mendonça‐Previato,
Tópico(s)Research on Leishmaniasis Studies
ResumoThe FASEB JournalVolume 13, Issue 12 p. 1627-1636 Research CommunicationsFree to Read Costimulatory action of glycoinositolphospholipids from Trypanosoma cruzi: increased interleukin 2 secretion and induction of nuclear translocation of the nuclear factor of activated T cells 1 Maria Bellio, Corresponding Author Maria Bellio [email protected] Instituto de Microbiologia Prof. Paulo de Goes, 21.941-590 Rio de Janeiro, RJ, Brazil Correspondence: Depto. de Imunologia/Instituto de Microbiologia Prof. Paulo de Goes, CCS Bl.I-2° andar Sala: I2-053, UFRJ -Cidade Universitaria, CEP: 21941-590 Ilha do Fundao, Rio de Janeiro, RJ. Brazil. E-mail: [email protected]Search for more papers by this authorAna-Carolina S. C. Oliveira, Ana-Carolina S. C. Oliveira Instituto de Microbiologia Prof. Paulo de Goes, 21.941-590 Rio de Janeiro, RJ, BrazilSearch for more papers by this authorClaudia S. Mermelstein, Claudia S. Mermelstein Departamento de Histologia e Embriologia, ICB, 21.941-590 Rio de Janeiro, RJ, BrazilSearch for more papers by this authorMarcia A. M. Capella, Marcia A. M. Capella Instituto de Biofísica Carlos Chagas F°, Universidade Federal do Rio de Janeiro, 21.941-590 Rio de Janeiro, RJ, BrazilSearch for more papers by this authorJoão P. B. Viola, João P. B. Viola Programa de Medicina Experimental, Instituto Nacional do Cancer, Rio de Janeiro, RJ, BrazilSearch for more papers by this authorJean-Pierre Levraud, Jean-Pierre Levraud Unité INSERM U 277, Institut Pasteur, 75724 Paris Cedex 15, FranceSearch for more papers by this authorGeorge A. Dosreis, George A. Dosreis Instituto de Biofísica Carlos Chagas F°, Universidade Federal do Rio de Janeiro, 21.941-590 Rio de Janeiro, RJ, BrazilSearch for more papers by this authorJosé O. Previato, José O. Previato Instituto de Microbiologia Prof. Paulo de Goes, 21.941-590 Rio de Janeiro, RJ, BrazilSearch for more papers by this authorLucia Mendonça-Previato, Lucia Mendonça-Previato Instituto de Microbiologia Prof. Paulo de Goes, 21.941-590 Rio de Janeiro, RJ, BrazilSearch for more papers by this author Maria Bellio, Corresponding Author Maria Bellio [email protected] Instituto de Microbiologia Prof. Paulo de Goes, 21.941-590 Rio de Janeiro, RJ, Brazil Correspondence: Depto. de Imunologia/Instituto de Microbiologia Prof. Paulo de Goes, CCS Bl.I-2° andar Sala: I2-053, UFRJ -Cidade Universitaria, CEP: 21941-590 Ilha do Fundao, Rio de Janeiro, RJ. Brazil. E-mail: [email protected]Search for more papers by this authorAna-Carolina S. C. Oliveira, Ana-Carolina S. C. Oliveira Instituto de Microbiologia Prof. Paulo de Goes, 21.941-590 Rio de Janeiro, RJ, BrazilSearch for more papers by this authorClaudia S. Mermelstein, Claudia S. Mermelstein Departamento de Histologia e Embriologia, ICB, 21.941-590 Rio de Janeiro, RJ, BrazilSearch for more papers by this authorMarcia A. M. Capella, Marcia A. M. Capella Instituto de Biofísica Carlos Chagas F°, Universidade Federal do Rio de Janeiro, 21.941-590 Rio de Janeiro, RJ, BrazilSearch for more papers by this authorJoão P. B. Viola, João P. B. Viola Programa de Medicina Experimental, Instituto Nacional do Cancer, Rio de Janeiro, RJ, BrazilSearch for more papers by this authorJean-Pierre Levraud, Jean-Pierre Levraud Unité INSERM U 277, Institut Pasteur, 75724 Paris Cedex 15, FranceSearch for more papers by this authorGeorge A. Dosreis, George A. Dosreis Instituto de Biofísica Carlos Chagas F°, Universidade Federal do Rio de Janeiro, 21.941-590 Rio de Janeiro, RJ, BrazilSearch for more papers by this authorJosé O. Previato, José O. Previato Instituto de Microbiologia Prof. Paulo de Goes, 21.941-590 Rio de Janeiro, RJ, BrazilSearch for more papers by this authorLucia Mendonça-Previato, Lucia Mendonça-Previato Instituto de Microbiologia Prof. Paulo de Goes, 21.941-590 Rio de Janeiro, RJ, BrazilSearch for more papers by this author First published: 01 September 1999 https://doi.org/10.1096/fasebj.13.12.1627Citations: 12Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat ABSTRACT The effects of the glycoinositolphospholipids (GIPLs) from Trypanosoma cruzi on T lymphocyte activation were investigated in a mouse T cell hybridoma (DO-11.10). Purified GIPLs from T. cruzi strains Y and G markedly increased IL-2 mRNA transcripts and IL-2 secretion induced by mitogenic anti-CD3 and anti-Thy1 mAbs. This costimulatory function was also revealed by the induction of IL-2 secretion after the simultaneous addition of the T. cruzi GIPLs and either the calcium ionophore A23187 or phorbol ester. The capacity of the GIPL molecule to induce an increase in cytoplasmic calcium levels was also demonstrated. After exposure of T cell hybridoma to GIPL, the nuclear transcription factor NFAT1 became partially dephosphory-lated, and its nuclear localization was demonstrated both in the T cell hybridoma and in Balb/c CD3+ cells. These results demonstrate that T. cruzi GIPL molecules are capable of signaling to T cells and therefore could be valuable tools for the study of T cell activation, besides playing a potential role in subverting the T lymphocyte immune response during T. cruzi infection.—Bellio, M., Oliveira, A.-C. S. C., Mermelstein, C. S., Capella, M. A. M., Viola, J. P. B., Levraud, J.-P., Dosreis, G. A., Pre-viato, J. O., Mendonça-Previato, L. Costimulatory action of glycoinositolphospholipids from Trypano-soma cruzi: increased interleukin 2 secretion and induction of nuclear translocation of the nuclear factor of activated T cells 1. FASEB J. 13, 1627–1636 (1999) REFERENCES 1McConville, M., and Ferguson, M. A. J. (1993) The structure, biosynthesis and function of glycosylated phosphatidylinositols in the parasitic protozoa and higher eukaryotes. Biochem. J. 294, 305–324 10.1042/bj2940305 CASPubMedWeb of Science®Google Scholar 2Previato, J. O., Gorin, P. A. J., Mazurek, M., Xavier, M. 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