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Biochemical and genetic analysis of the Drk SH2/SH3 adaptor protein of Drosophila.

1995; Springer Nature; Volume: 14; Issue: 11 Linguagem: Inglês

10.1002/j.1460-2075.1995.tb07248.x

1460-2075

Thomas Raabe, Jean Paul Olivier, Barry J. Dickson, X. Johné Liu, Gerald Gish, Tony Pawson, Ernst Hafen,

Plant Reproductive Biology

Research Article1 June 1995free access Biochemical and genetic analysis of the Drk SH2/SH3 adaptor protein of Drosophila. T. Raabe T. Raabe Zoologisches Institut, Universität Zürich, Switzerland. Search for more papers by this author J.P. Olivier J.P. Olivier Zoologisches Institut, Universität Zürich, Switzerland. Search for more papers by this author B. Dickson B. Dickson Zoologisches Institut, Universität Zürich, Switzerland. Search for more papers by this author X. Liu X. Liu Zoologisches Institut, Universität Zürich, Switzerland. Search for more papers by this author G.D. Gish G.D. Gish Zoologisches Institut, Universität Zürich, Switzerland. Search for more papers by this author T. Pawson T. Pawson Zoologisches Institut, Universität Zürich, Switzerland. Search for more papers by this author E. Hafen E. Hafen Zoologisches Institut, Universität Zürich, Switzerland. Search for more papers by this author T. Raabe T. Raabe Zoologisches Institut, Universität Zürich, Switzerland. Search for more papers by this author J.P. Olivier J.P. Olivier Zoologisches Institut, Universität Zürich, Switzerland. Search for more papers by this author B. Dickson B. Dickson Zoologisches Institut, Universität Zürich, Switzerland. Search for more papers by this author X. Liu X. Liu Zoologisches Institut, Universität Zürich, Switzerland. Search for more papers by this author G.D. Gish G.D. Gish Zoologisches Institut, Universität Zürich, Switzerland. Search for more papers by this author T. Pawson T. Pawson Zoologisches Institut, Universität Zürich, Switzerland. Search for more papers by this author E. Hafen E. Hafen Zoologisches Institut, Universität Zürich, Switzerland. Search for more papers by this author Author Information T. Raabe1, J.P. Olivier1, B. Dickson1, X. Liu1, G.D. Gish1, T. Pawson1 and E. Hafen1 1Zoologisches Institut, Universität Zürich, Switzerland. The EMBO Journal (1995)14:2509-2518https://doi.org/10.1002/j.1460-2075.1995.tb07248.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The Drk SH3-SH2-SH3 adaptor protein has been genetically identified in a screen for rate-limiting components acting downstream of the Sevenless (Sev) receptor tyrosine kinase in the developing eye of Drosophila. It provides a link between the activated Sev receptor and Sos, a guanine nucleotide release factor that activates Ras1. We have used a combined biochemical and genetic approach to study the interactions between Sev, Drk and Sos. We show that Tyr2546 in the cytoplasmic tail of Sev is required for Drk binding, probably because it provides a recognition site for the Drk SH2 domain. Interestingly, a mutation at this site does not completely block Sev function in vivo. This may suggest that Sev can signal in a Drk-independent, parallel pathway or that Drk can also bind to an intermediate docking protein. Analysis of the Drk-Sos interaction has identified a high affinity binding site for Drk SH3 domains in the Sos tail. We show that the N-terminal Drk SH3 domain is primarily responsible for binding to the tail of Sos in vitro, and for signalling to Ras in vivo. Previous ArticleNext Article Volume 14Issue 111 June 1995In this issue RelatedDetailsLoading ...