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Leukocyte Inhibitory Factor (LIF): Production by Purified Human T and B Lymphocytes

1975; American Association of Immunologists; Volume: 115; Issue: 1 Linguagem: Inglês

10.4049/jimmunol.115.1.315

1550-6606

Leonard Chess, Ross E. Rocklin, Richard P. MacDermott, John R. David, Stuart F. Schlossman,

Nuclear Receptors and Signaling

Abstract Using column immunoabsorbent chromatography to isolate highly purified subpopulations of human lymphocytes, we have previously shown that T cells, but not B cells, are specifically triggered by antigens to proliferate in vitro (1, 2). Although B cells were not triggered to proliferate, they, like T cells, were specifically activated by soluble antigens to secrete migration inhibitory factor (MIF)3 (3). These initial studies prompted further investigations which showed that: 1) B cells produced quantitatively more MIF than did T cells, 2) B cell MIF is indistinguishable from T cell MIF by Sephadex G-100 chromatography, and 3) B cell MIF production, unlike T cell MIF production, was unaffected by BUdR and light, agents known to inhibit the proliferative response (3). Recently, another mediator of cellular immunity, leukocyte inhibitory factor (LIF), has been described (4). This material, which is distinct from MIF, inhibits the migration of human polymorphonuclear leukocytes (PMN), but not human monocytes or guinea pig macrophages.