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Transglycosylic Reactions of Nucleotides of Deoxy Sugars

1976; Wiley; Volume: 64; Issue: 1 Linguagem: Inglês

10.1111/j.1432-1033.1976.tb10298.x

1432-1033

Jiří Zemek, J. Strmeň, Štefan. KULČÁR, Štefan Bauer,

Microbial Metabolic Engineering and Bioproduction

The biosynthesis of alpha,alpha'-trehalose was investigated in a reaction catalyzed with yeast UDP-Glc :glucose-6-phosphate 1-glucosyl transferase. It was revealed that the hydroxyls at positions C-2, C-3, C-4 and C-6 are not essential for preserving its substrate properties in this reaction. The affinity of the enzyme and the rate of hexose incorporation into trehalose decreases in following sequence: UDP-glucose, UDP-2-deoxy-D-glucose, UDP-6-deoxy-d-glucose, UDP-4-deoxy-D-glucose and UDP-3-deoxy-D-glucose.