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Interferon Production in Microcarrier Culture of Human Fibroblast Cells

1984; Springer Nature; Linguagem: Inglês

10.1007/978-1-4615-9376-8_10

2214-8019

V. G. Edy,

RNA Interference and Gene Delivery

The microcarrier cell culture technique offers a convenient way of manipulating relatively large amounts of anchorage-dependent cultured mammalian cells in a relatively small volume. Other advantages of this system are ease of direct observation, and of monitoring and control of other culture parameters. The production of human fibroblasts interferon on microcarrier culture on a scale of up to 44 litres (very roughly equivalent to 1100 roller bottles) using a conventional inducation with poly(I) poly(C), followed by superinduction by treatment with metalolic inhibitors, is described.