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Acetylene Reduction (Nitrogen Fixation) by Pulp and Paper Mill Effluents and by Klebsiella Isolated from Effluents and Environmental Situations

1974; American Society for Microbiology; Volume: 28; Issue: 4 Linguagem: Inglês

10.1128/am.28.4.608-613.1974

0003-6919

Roger Knowles, R. Neufeld, Stuart L. Simpson,

Enzyme-mediated dye degradation

High rates of acetylene (C 2 H 2 ) reduction (nitrogenase activity) were observed in woodroom effluent from a neutral sulfite semi-chemical mill under aerobic (up to 644 nmol of C 2 H 4 produced per ml per h) and under anaerobic (up to 135 nmol of C 2 H 4 produced per ml per h) conditions. Pasteurized effluent developed C 2 H 2 reduction activity when incubated under anaerobic but not under aerobic conditions. Activities were increased by addition of 0.5 to 3.0% glucose or xylose. Enrichment and enumeration studies showed that N 2 -fixing Azotobacter and Klebsiella were abundant, and N 2 -fixing Bacillus was present. Of 129 isolates of Klebsiella from pulp mills, lakes, rivers, and drainage and sewage systems, 32% possessed nitrogen-fixing ability.