Artigo Acesso aberto Revisado por pares

Heparinase treatment of RNA before quantitative real-time RT-PCR

2003; Future Science Ltd; Volume: 35; Issue: 6 Linguagem: Inglês

10.2144/03356bm03

ISSN

1940-9818

Autores

Mary L. Johnson, C. Navanukraw, Anna T. Grazul‐Bilska, Lawrence P. Reynolds, Dale A. Redmer,

Tópico(s)

Molecular Biology Techniques and Applications

Resumo

BioTechniquesVol. 35, No. 6 BenchmarksOpen AccessHeparinase treatment of RNA before quantitative real-time RT-PCRMary Lynn Johnson, Chainarong Navanukraw, Anna T. Grazul-Bilska, Lawrence P. Reynolds & Dale A. RedmerMary Lynn Johnson*Address correspondence to Mary Lynn Johnson, North Dakota State University, Department of Animal and Range Sciences, Fargo, ND, USA. e-mail: E-mail Address: mary.lynn.johnson@ndsu.nodak.eduNorth Dakota State University, Fargo, ND, USA, Chainarong NavanukrawNorth Dakota State University, Fargo, ND, USA, Anna T. Grazul-BilskaNorth Dakota State University, Fargo, ND, USA, Lawrence P. ReynoldsNorth Dakota State University, Fargo, ND, USA & Dale A. RedmerNorth Dakota State University, Fargo, ND, USAPublished Online:24 Sep 2018https://doi.org/10.2144/03356bm03AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInReddit FiguresReferencesRelatedDetailsCited ByCirculating microRNAs as Reliable Tumor Biomarkers: Opportunities and Challenges Facing Clinical Application9 July 2022 | Journal of Pharmacology and Experimental Therapeutics, Vol. 384, No. 1Production, characteristics and applications of microbial heparinasesBiochimie, Vol. 198Techniques for RNA extraction from cells cultured in starPEG–heparin hydrogels2 June 2021 | Open Biology, Vol. 11, No. 6Peripheral blood microRNAs and the COVID-19 patient: methodological considerations, technical challenges and practice points15 February 2021 | RNA Biology, Vol. 18, No. 5MicroRNA in dried blood spots from patients with Aagenaes syndrome and evaluation of pre-analytical and analytical factors15 September 2020 | Pediatric Research, Vol. 89, No. 7The prognostic impact of circulating miRNAs in patients with advanced esophagogastric cancer during palliative chemotherapyCancer Treatment and Research Communications, Vol. 27MicroRNA-331 and microRNA-151-3p as biomarkers in patients with ST-segment elevation myocardial infarction3 April 2020 | Scientific Reports, Vol. 10, No. 1T‐cells producing multiple combinations of IFNγ, TNF and IL10 are associated with mild forms of dengue infection6 April 2020 | Immunology, Vol. 160, No. 1Diets enriched in red seaweed (Pyropia columbina and Gracilaria chilensis) cryo concentrates modulate the immune-relevant gene encoding the Mx antiviral protein in salmon (Salmo salar) white blood cells7 August 2018 | Journal of Applied Phycology, Vol. 31, No. 2Endogenous Heparin Interferes with Quantification of MicroRNAs by RT-qPCR1 May 2018 | Clinical Chemistry, Vol. 64, No. 5Capacity of rTth polymerase to detect RNA in the presence of various inhibitors2 January 2018 | PLOS ONE, Vol. 13, No. 1Newly discovered hepatitis C virus minicores circulate in human blood12 November 2017 | Hepatology Communications, Vol. 2, No. 1Modified high-throughput quantification of plasma microRNAs in heparinized patients with coronary artery disease using heparinaseBiochemical and Biophysical Research Communications, Vol. 493, No. 1Pre-examination factors affecting molecular diagnostic test results and interpretation: A case-based approachClinica Chimica Acta, Vol. 467The Clinical Potential of Heart Failure–Related miRNAsPhylogenetic analysis of full-length, early infection, hepatitis C virus genomes among people with intravenous drug use: the InC 3 Study3 November 2016 | Journal of Viral Hepatitis, Vol. 24, No. 1Amplification-Free Detection of Circulating microRNA Biomarkers from Body Fluids Based on Fluorogenic Oligonucleotide-Templated Reaction between Engineered Peptide Nucleic Acid Probes: Application to Prostate Cancer Diagnosis7 August 2016 | Analytical Chemistry, Vol. 88, No. 16Heparinase treatment of heparin-contaminated plasma from coronary artery bypass grafting patients enables reliable quantification of microRNAsBiomolecular Detection and Quantification, Vol. 8Avoiding heparin inhibition in circulating MicroRNAs amplificationInternational Journal of Cardiology, Vol. 207Optimized Collection Protocol for Plasma MicroRNA Measurement in Patients with Cardiovascular DiseaseBioMed Research International, Vol. 2016Differential expression of plasma microRNA in preeclamptic patients at delivery and 1 year postpartumAmerican Journal of Obstetrics and Gynecology, Vol. 213, No. 3Transcriptome-wide measurement of ribosomal occupancy by ribosome profilingMethods, Vol. 85Optimized Treatment of Heparinized Blood Fractions to Make Them Suitable for AnalysisBiopreservation and Biobanking, Vol. 13, No. 4Elimination of heparin interference during microarray processing of fresh and biobank-archived blood samples17 April 2014 | Environmental and Molecular Mutagenesis, Vol. 55, No. 6Issues and Prospects of microRNA-Based Biomarkers in Blood and Other Body Fluids14 May 2014 | Molecules, Vol. 19, No. 5Evaluation of carrier-mediated siRNA delivery: Lessons for the design of a stem-loop qPCR-based approach for quantification of intracellular full-length siRNAJournal of Controlled Release, Vol. 166, No. 3MicroRNA Detection in Plasma SamplesThe Journal of Molecular Diagnostics, Vol. 15, No. 1Standardization of sample collection, isolation and analysis methods in extracellular vesicle research27 May 2013 | Journal of Extracellular Vesicles, Vol. 2, No. 1Analysis of MicroRNA Niches: Techniques to Measure Extracellular MicroRNA and Intracellular MicroRNA In Situ5 May 2013miRNA expression profiling for identification of potential breast cancer biomarkers17 May 2012 | Biomarkers, Vol. 17, No. 5Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA QuantitationThe Journal of Molecular Diagnostics, Vol. 14, No. 1Heparin Elevates Circulating Soluble fms-Like Tyrosine Kinase-1 Immunoreactivity in Pregnant Women Receiving Anticoagulation TherapyCirculation, Vol. 124, No. 23Inhibition of Taq polymerase as a method for screening heparin for oversulfated contaminantsBiomaterials, Vol. 29, No. 36Development of an Assay to Determine Single Nucleotide Polymorphisms in the Prion Gene for the Genetic Diagnosis of Relative Susceptibility to Classical Scrapie in Sheep25 June 2016 | Journal of Veterinary Diagnostic Investigation, Vol. 19, No. 1 Vol. 35, No. 6 Follow us on social media for the latest updates Metrics Downloaded 491 times History Published online 24 September 2018 Published in print December 2003 Information© 2018 Author(s)PDF download

Referência(s)