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Production and Characterization of an Exopolysaccharide Excreted by a Deep-Sea Hydrothermal Vent Bacterium Isolated from the Polychaete Annelid Alvinella pompejana

1994; American Society for Microbiology; Volume: 60; Issue: 11 Linguagem: Inglês

10.1128/aem.60.11.4134-4141.1994

1098-5336

P Vincent, Patricia Pignet, Franck Talmont, L Bozzi, Bernard Fournet, Jean Guézennec, Christian Jeanthon, Daniël Prieur,

Microbial Fuel Cells and Bioremediation

The heterotrophic and mesophilic marine bacterium HYD-1545 was isolated on a metal-amended medium from the dorsal integument of the hydrothermal vent polychaete Alvinella pompejana . This strain, which can be assigned to the genus Alteromonas on the basis of its G+C content and phenotypical features, produced large amounts of an acidic polysaccharide in batch cultures. The polysaccharide was excreted during the stationary phase of growth and contained glucose, galactose, glucuronic acid, galacturonic acid, and 4,6-O-(1-carboxyethilidene)-galactose as major components. This polysaccharide was a polyelectrolyte, and the viscosity of its solutions depended on the ionic strength. The decrease in viscosity with increasing NaCl concentrations and the effect of Ca 2+ in decreasing the viscosity at low Ca 2+ concentrations support a model in which the polysaccharide carries anionic groups. However, an unusual behavior was observed at higher concentrations and could be related to intermolecular interactions involving Ca 2+ ions.