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Phylogenetic relationships between isolated populations of the limestone‐dwelling microsnail Gyliotrachela hungerfordiana (Gastropoda: Vertiginidae)

2011; Wiley; Volume: 49; Issue: 4 Linguagem: Inglês

10.1111/j.1439-0469.2011.00623.x

1439-0469

Paul H. Hoekstra, Menno Schilthuizen,

Marine Biology and Ecology Research

The vertiginid species Gyliotrachela hungerfordiana, an obligate limestone-dwelling microsnail, is relatively widespread and is found on a large number of isolated limestone hills in Peninsular Malaysia. To elucidate the pattern of colonization of these hills, we conducted a molecular phylogenetic analysis on G. hungerfordiana subpopulations from 15 separate limestone outcrops. As outgroups, we also included five related Peninsular Malaysian Vertiginidae (Gyliotrachela frequens, Gyliotrachela transitans, Gyliotrachela salpinx, Gyliotrachela depressispira and Paraboysidia tarutao), one population each. A combined analysis of nuclear (internal transcribed spacer 1) and mitochondrial (cytochrome c oxidase 1) sequences showed that (1) G. hungerfordiana is monophyletic; (2) there is a clear geographical pattern in the phylogenetic relationships between G. hungerfordiana populations, with genetic distances increasing with geographic distance; (3) this pattern is disturbed by a few long-distance (north-west to south-east and north to south) colonizations. La especie Gyliotrachela hungerfordiana de la familia Vertiginidae, un micromolusco que vive obligatoriamente en piedras calizas, tiene una distribución relativamente amplia y puede ser hallada en un gran número de colinas de piedra caliza aisladas en la península de Malasia. Para dilucidar el patrón de colonización de la especie en estas colinas, realizamos un análisis filogenético molecular de 15 sub-poblaciones de G. hungerfordiana separadas por afloramientos de piedra caliza. Como grupos externos, también incluimos una población para cada una de las cinco especies (G. frequens, G. transitans, G. salpinx, G. depressispira y Paraboysidia tarutao) de la familia Vertiginidae de la península de Malasia relacionados con G. hungerfordiana. El análisis combinado de secuencias nucleares (Internal Transcribed Spacer 1; ITS-1) y mitocondriales (Cytochrome C Oxidase 1; COI) mostró lo siguiente: (1) G. hungerfordiana es monofilética; (2) existe un patrón geográfico claro de las relaciones filogenéticas entre las poblaciones de G. hungerfordiana, con un incremento de las distancias genéticas a mayor distancia geográfica; (3) el patrón anterior es interrumpido por algunas colonizaciones de larga distancia (noroeste a sureste y de norte a sur). Table S1. A list of all sequences analysed in this study, including taxonomic identities, collection localities (numbers refer to Fig. 1) and Genbank accession numbers. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.