Portal de Periódicos da CAPES

Airway smooth muscle relaxation results from a reduction in the frequency of Ca2+ oscillations induced by a cAMP-mediated inhibition of the IP3 receptor

2006; BioMed Central; Volume: 7; Issue: 1 Linguagem: Inglês

10.1186/1465-9921-7-34

1465-993X

Yan Bai, Michael J. Sanderson,

Neuropeptides and Animal Physiology

Abstract Background It has been shown that the contractile state of airway smooth muscle cells (SMCs) in response to agonists is determined by the frequency of Ca 2+ oscillations occurring within the SMCs. Therefore, we hypothesized that the relaxation of airway SMCs induced by agents that increase cAMP results from the down-regulation or slowing of the frequency of the Ca 2+ oscillations. Methods The effects of isoproterenol (ISO), forskolin (FSK) and 8-bromo-cAMP on the relaxation and Ca 2+ signaling of airway SMCs contracted with methacholine (MCh) was investigated in murine lung slices with phase-contrast and laser scanning microscopy. Results All three cAMP-elevating agents simultaneously induced a reduction in the frequency of Ca 2+ oscillations within the SMCs and the relaxation of contracted airways. The decrease in the Ca 2+ oscillation frequency correlated with the extent of airway relaxation and was concentration-dependent. The mechanism by which cAMP reduced the frequency of the Ca 2+ oscillations was investigated. Elevated cAMP did not affect the re-filling rate of the internal Ca 2+ stores after emptying by repetitive exposure to 20 mM caffeine. Neither did elevated cAMP limit the Ca 2+ available to stimulate contraction because an elevation of intracellular Ca 2+ concentration induced by exposure to a Ca 2+ ionophore (ionomycin) or by photolysis of caged-Ca 2+ did not reverse the effect of cAMP. Similar results were obtained with iberiotoxin, a blocker of Ca 2+ -activated K + channels, which would be expected to increase Ca 2+ influx and contraction. By contrast, the photolysis of caged-IP 3 in the presence of agonist, to further elevate the intracellular IP 3 concentration, reversed the slowing of the frequency of the Ca 2+ oscillations and relaxation of the airway induced by FSK. This result implied that the sensitivity of the IP 3 R to IP 3 was reduced by FSK and this was supported by the reduced ability of IP 3 to release Ca 2+ in SMCs in the presence of FSK. Conclusion These results indicate that the relaxant effect of cAMP-elevating agents on airway SMCs is achieved by decreasing the Ca 2+ oscillation frequency by reducing internal Ca 2+ release through IP 3 receptors.