Isolation of a cDNA encoding a human serum marker for acute pancreatitis. Identification of pancreas-specific protein as pancreatic procarboxypeptidase B.
1992; Elsevier BV; Volume: 267; Issue: 4 Linguagem: Inglês
10.1016/s0021-9258(18)45919-4
ISSN1083-351X
AutoresKazuo Yamamoto, Åke Pousette, P Chow, Hildegarde Wilson, Saqib Shami, Cynthia K. French,
Tópico(s)Endoplasmic Reticulum Stress and Disease
ResumoA human pancreas-specific protein (PASP), previously characterized as a serum marker for acute pancreatitis and pancreatic graft rejection, has been identified as pancreatic procarboxypeptidase B (PCPB).cDNAs encoding PASP/PCPB were isolated from a human pancreas cDNA library using a combination of nucleic acid hybridization screening and immunoscreening with antisera raised against native PASP.The deduced amino acid sequence of PASPPCPB cDNA predicts the translation of a 416-amino acid preproenzyme with a 16-amino acid signalpeader peptide and a 96-amino acid activation peptide.The proenzyme portion of this protein has 76% identity with rat PCPB and 84% identity with bovine carboxypeptidase B. DNA and RNA blot analyses indicate that human PCPB mRNA (1,400 nucleotides) is transcribed from a single locus in the human genome in a tissue-specific fashion.N-terminal sequencing of native PASP and the specific immunoreactivity of bacterially expressed PASP/PCPB with native PASP antibodies confirm the identification of PASP as human pancreatic PCPB.
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