Coconut as a Medium for the Experimental Production of Aflatoxin
1969; American Society for Microbiology; Volume: 18; Issue: 1 Linguagem: Inglês
10.1128/am.18.1.88-94.1969
ISSN0003-6919
AutoresS. N. Arseculeratne, L. M. De Silva, S. Wijesundera, C. H. S. R. Bandunatha,
Tópico(s)Plant tissue culture and regeneration
ResumoFresh, grated coconut has been found to be an excellent medium for aflatoxin production by Aspergillus flavus . Under optimal conditions, yields of 8 mg of total aflatoxin per g of substrate were obtained. Continuous agitation of the growth medium under moist conditions at 24 C produced highest yields. Aflatoxin was assayed both biologically and chromatographically. The aflatoxin content of cultures varied biphasically with the duration of incubation. It is suggested that this pattern could result from the sequential operation of factors promoting aflatoxin formation on the one hand and a detoxifying mechanism on the other.
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