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Discovery and characterization of Coturnix chinensis avian β ‐defensin 10, with broad antibacterial activity

2012; Wiley; Volume: 18; Issue: 4 Linguagem: Inglês

10.1002/psc.1437

1099-1387

Deying Ma, Lijuan Lin, Kexin Zhang, Zongxi Han, Yuhao Shao, Ruiqin Wang, Shengwang Liu,

Biochemical and Structural Characterization

A novel avian β ‐defensin (AvBD), AvBD10, was discovered in the liver and bone marrow tissues from Chinese painted quail ( Coturnix chinensis ) in the present study. The complete nucleotide sequence of quail AvBD10 contains a 207‐bp open reading frame that encodes 68 amino acids. The quail AvBD10 was expressed widely in all the tissues from quails except the tongue, crop, breast muscle, and thymus and was highly expressed in the bone marrow. In contrast to the expression pattern of AvBD10 in tissues from quail, the chicken AvBD10 was expressed in all 21 tissues from the layer hens investigated, with a high level of expression in the kidney, lung, liver, bone marrow, and Harderian glands. Recombinant glutathione S‐transferase (GST)‐tagged AvBD10s of both quail and chicken were produced and purified by expression of the two cDNAs in Escherichia coli , respectively. In addition, peptide according to the respective AvBD10s sequence was synthesized, named synthetic AvBD10s. As expected, both recombinant GST‐tagged AvBD10s and synthetic AvBD10s of quail and chicken exhibited similar bactericidal properties against most bacteria, including Gram‐positive and Gram‐negative forms. However, no significant bactericidal activity was found for quail recombinant GST‐tagged AvBD10 against Salmonella choleraesuis or for chicken recombinant GST‐tagged AvBD10 against Proteus mirabilis . Copyright © 2012 European Peptide Society and John Wiley & Sons, Ltd.