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Differentiation between active and inactive human brucellosis by measuring antiprotein humoral immune responses

1992; American Society for Microbiology; Volume: 30; Issue: 3 Linguagem: Inglês

10.1128/jcm.30.3.604-607.1992

1098-660X

Fernando A. Goldbaum, Carlos P. Rubbi, Jorge C. Wallach, Silvia E. Miguel, Pablo C. Baldi, Carlos A. Fossati,

Galectins and Cancer Biology

A preparation of Brucella abortus cytoplasmic proteins was depleted of lipopolysaccharide (LPS) by immunoadsorption with a monoclonal antibody (MAb), BC68, specific for the O antigen of B. abortus smooth LPS. Two enzyme-linked immunosorbent assay (ELISA) systems were developed and used in this study. The first system includes an LPS-free cytoplasmic protein preparation; the second one was based on antigen capture on MAb BC68. By using these systems, we have demonstrated that 94% (33 of 35) of the brucellosis patients studied showed immunoglobulin G antiprotein response and also that all of the patients showed a strong anti-LPS reactivity. Thirty-six serologically positive individuals with no active infection at the time of examination (SPI) were also included. No immunoglobulin G antibodies against proteins were detected in 34 of them (92%), whereas 31 SPI (86%) showed various degrees of anti-LPS reactivity. The use of the LPS-free protein extract in ELISAs made it possible to establish differential reactivity patterns between active and inactive brucellosis.