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Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil

2015; Instituto Oswaldo Cruz, Ministério da Saúde; Volume: 110; Issue: 5 Linguagem: Inglês

10.1590/0074-02760150053

1678-8060

Jefferson Santos, Tereza Magalhães, José Valter Joaquim Silva Júnior, Andréa Nazaré Monteiro Rangel da Silva, Marli Tenório Cordeiro, Laura H. V. G. Gil,

Malaria Research and Control

Full-length dengue virus (DENV) cDNA clones are an invaluable tool for many studies, including those on the development of attenuated or chimeric vaccines and on host-virus interactions. Furthermore, the importance of low passage DENV infectious clones should be highlighted, as these may harbour critical and unique strain-specific viral components from field-circulating isolates. The successful construction of a functional Brazilian low passage DENV serotype 2 full-length clone through homologous recombination reported here supports the use of a strategy that has been shown to be highly useful by our group for the development of flavivirus infectious clones and replicons.