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Physico-chemical properties and protein profiles of sperm from three freshwater chondrostean species: a comparative study among Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula)

2011; Wiley; Volume: 27; Issue: 2 Linguagem: Inglês

10.1111/j.1439-0426.2010.01634.x

1439-0426

P. Li, Marek Rodina, Martin Hulák, David Gela, Martin Pšenička, Z. H. Li, Otomar Linhart,

Fish Biology and Ecology Studies

Journal of Applied IchthyologyVolume 27, Issue 2 p. 673-677 Physico-chemical properties and protein profiles of sperm from three freshwater chondrostean species: a comparative study among Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula) P. Li, P. Li South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Jingzhou, Hubei, ChinaSearch for more papers by this authorM. Rodina, M. Rodina South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech RepublicSearch for more papers by this authorM. Hulak, M. Hulak South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech RepublicSearch for more papers by this authorD. Gela, D. Gela South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech RepublicSearch for more papers by this authorM. Psenicka, M. Psenicka South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech RepublicSearch for more papers by this authorZ. H. Li, Z. H. Li South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Jingzhou, Hubei, ChinaSearch for more papers by this authorO. Linhart, O. Linhart South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech RepublicSearch for more papers by this author P. Li, P. Li South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Jingzhou, Hubei, ChinaSearch for more papers by this authorM. Rodina, M. Rodina South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech RepublicSearch for more papers by this authorM. Hulak, M. Hulak South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech RepublicSearch for more papers by this authorD. Gela, D. Gela South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech RepublicSearch for more papers by this authorM. Psenicka, M. Psenicka South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech RepublicSearch for more papers by this authorZ. H. Li, Z. H. Li South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Jingzhou, Hubei, ChinaSearch for more papers by this authorO. Linhart, O. Linhart South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech RepublicSearch for more papers by this author First published: 28 March 2011 https://doi.org/10.1111/j.1439-0426.2010.01634.xCitations: 13 Author's address: P. Li, University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, 389 25 Vodnany, Czech Republic.E-mail: [email protected] Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Summary Physico-chemical properties (spermatozoa motility, spermatozoa concentration, seminal plasma protein concentration, osmolality, pH, ions) and sperm protein profiles were evaluated in three Acipenseriforme species [Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula)]. The spermatozoa velocity was observed significantly highest in sterlet and lowest in Siberian sturgeon (P < 0.05). Spermatozoa velocity was positively correlated to pH and the concentration of Na+ in seminal plasma in all species examined. Other tested parameters, in particular the concentration of K+, expressed species specific patterns, which could be linked to taxonomical status of species. The protein profiles of seminal plasma indicated a protein band with a molecular weight of 32.6 kDa exclusively detected in paddlefish. In spermatozoa, 95 protein spots were detected in Siberian sturgeon and sterlet, respectively, whereas only 70 spots were observed in paddlefish. Each spermatozoa protein map expressed a strong species specific pattern, which offers a basic benchmark to trace molecular differences among Acipenseriformes. It can be concluded that results presented in this study could be applied for successful reproduction management and cryopreservation protocols of these endangered species. References Alavi, S. M. H.; Cosson, J., 2006: Sperm motility in fishes: (II) Effects of ions and osmotic pressure. Cell Biol. Int. 30, 1– 14. Alavi, S. M. H.; Linhart, O.; Coward, K.; Rodina, M., 2008: Fish spermatology: implications for aquaculture management. In: Fish spermatology. S. M. H. Alavi, J. J. Cosson, K. Coward, G. Rafiee (Eds), Alpha science Ltd, Oxford, pp. 397– 460. 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