Portal de Periódicos da CAPES

High‐affinity immunoglobulin E receptor (FcεRI)‐bearing eosinophils, mast cells, macrophages and Langerhans’ cells in allergen‐induced late‐phase cutaneous reactions in atopic subjects

1998; Wiley; Volume: 93; Issue: 2 Linguagem: Inglês

10.1046/j.1365-2567.1998.00418.x

1365-2567

Ying Ying, BARATA, Meng Meng, Grant, Barkans, Durham, Kay,

Allergic Rhinitis and Sensitization

We have used in situ hybridization (ISH) and immunohistochemistry (IHC) to investigate the kinetics of the expression for FcεRI mRNA (α‐, β‐ and γ‐chains), the α‐chain protein product, as well as the phenotype of the mRNA‐ or protein‐positive cells in allergen‐induced late‐phase skin reactions in atopic subjects. Compared with diluent controls, there were significant increases in the total numbers of mRNA + cells for the α‐, β‐and γ‐chains for FcεRI at all time‐points (6, 24 and 48 hr) after allergen challenge ( P <0·01). By double IHC/ISH significant increases in α‐, β‐ and γ‐chain mRNA + macrophages, eosinophils, mast cells and CD1a + cells were also observed after allergen challenge ( P <0·05). The distribution of FcεRI subunit (α‐, β‐, or γ‐chain) mRNA + co‐localization was CD68 + macrophages (42–47%), EG2 + eosinophils (33–39%), tryptase + mast cells (5–11%) and CD1a + Langerhans’ cells (2–4%). Using single IHC, significant increases in the total number of FcεRI protein + cells ( P <0·01) were observed 24 and 48 hr after allergen challenge. Double IHC showed that the distribution of FcεRI + cells was tryptase + mast cells (33%), CD68 + macrophages (36%), EG2 + eosinophils (20%), CD1a + Langerhans’ cells (4%) and unidentified cells (7%), at the 24‐hr allergen‐challenged sites. These observations suggest that the cutaneous late‐phase reaction in man is associated with up‐regulation of FcεRI on eosinophils, macrophages, mast cells and Langerhans’ cells.