CHEMICAL AND PHYSICAL STUDIES ON THE ANTIHEMORRHAGIC VITAMIN
1937; Elsevier BV; Volume: 117; Issue: 2 Linguagem: Inglês
10.1016/s0021-9258(18)74552-3
ISSN1083-351X
Autores Tópico(s)Various Chemistry Research Topics
ResumoA description of methods used in concentrating the antihemorrhagic vitamin of the chick was given in preceding papers (1, 2).Since the vitamin had been purified to such extent that 0.5 microgram of the concentrate per gm. of the diet was capable of meeting the requirements of the chick for this factor, it was believed that chemical and physical studies could be made to throw further light on its nature.The procedure for preventive assay has been already explained (l-3).The methods and results of the present studies are described below.In all cases the concentrate used was the yellow, viscous oil obtained by molecular distillation (2). Methods and ResultsNitrogen-Samples of 24 mg.gave results by the micro-Kjeldahl method (Pregl) which indicated a nitrogen content of 0.23 per cent.' Sulfur-Two samples of 9 mg.each were tested for sulfur by the sodium fusion-plumbate method.The tests were negative.Two samples of similar weight were fused in a platinum dish with a mixture of 6 parts of sodium carbonate and 1 part of potassium chlorate.The fused mass was dissolved in distilled water, filtered, acidified with hydrochloric acid, and tested for sulfate with barium chloride.No barium sulfate was precipitated.The concentrate contained no detectable amount of sulfur.Phosphorus-A sample of 9.5 mg. was heated with the carbonatechlorate mixture in a platinum dish.When all traces of carbon 1 The author is indebted to Professor D. M.
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