Incidencia de patulina en jugo de manzana industrializado
1996; EDITIONS PUBLICATIONS ALIMENTARIAS SA; Issue: 276 Linguagem: Inglês
ISSN
0300-5755
AutoresM. Manchisky, Antônio Flávio Mı́dio,
Tópico(s)Mycotoxins in Agriculture and Food
Resumo73 samples of apple juice commercially available on the market of southern Brazil were analyzed to detect their patulin contents. The extraction of the mycotoxin was performed with ethylacetate and liquid-liquid partition with 1.5% sodium carbonate. The identification and quantification of patulin weas achieved by HPLC technique in a C 18 reversed phase column and 1.2% tetrahydrofuran in 0.02% sodium azida in water as the solvent. The method showed a recovery of 9%. The limit of detection (LOD) was ng and the limit of quantification (LOQ) 3 ng. The average percentage of contamination of the samples was 21 and the concentration range varied from 6.4 to 77 μg/l. These results should alert Brazilian health authorities to establish a maximal permissible level (MPL) or patulin in apple juice as well as in some other fruit commodities.
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