Portal de Periódicos da CAPES

The coxsackievirus A9 RGD motif is not essential for virus viability

1995; American Society for Microbiology; Volume: 69; Issue: 12 Linguagem: Inglês

10.1128/jvi.69.12.8035-8040.1995

1098-5514

Pamela Hughes, C. Horsnell, Timo Hyypiä, Glyn Stanway,

Cytomegalovirus and herpesvirus research

An RGD (arginine-glycine-aspartic acid) motif in coxsackievirus A9 has been implicated in internalization through an interaction with the integrin alpha v beta 3. We have produced a number of virus mutants, lacking the motif, which have a small-plaque phenotype in LLC-Mk2 and A-Vero cells and are phenotypically normal in RD cells. Substitution of flanking amino acids also affected plaque size. The results suggest that interaction between the RGD motif and alpha v beta 3 is not critical for virus viability in the cell lines tested and therefore that alternative regions of the CAV-9 capsid are involved in internalization.