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Simultaneous Detection of Bacteroides forsythus and Prevotella intermedia by 16S rRNA Gene-Directed Multiplex PCR

1999; American Society for Microbiology; Volume: 37; Issue: 5 Linguagem: Inglês

10.1128/jcm.37.5.1621-1624.1999

1098-660X

Georg Conrads, Daniel S. Thoma, I. Seyfarth, Friedrich Lampert, Rudolf Lütticken,

Infective Endocarditis Diagnosis and Management

ABSTRACT In a 16S rRNA gene-directed multiplex PCR, Prevotella intermedia - and Bacteroides forsythus -specific reverse primers were combined with a single conserved forward primer. A 660-bp fragment and an 840-bp fragment that were specific for both species could be amplified simultaneously. A total of 152 clinical samples, subgingival plaque and swabs of three different oral mucosae, from 38 periodontitis patients were used for the evaluation.