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Complex asparagine-linked oligosaccharides are required for morphogenic events during post-implantation development.

1994; Springer Nature; Volume: 13; Issue: 9 Linguagem: Inglês

10.1002/j.1460-2075.1994.tb06480.x

1460-2075

Martina Metzler, A. Gertz, Mohan Sarkar, Harry Schachter, John W. Schrader, Jamey D. Marth,

Galectins and Cancer Biology

Research Article1 May 1994free access Complex asparagine-linked oligosaccharides are required for morphogenic events during post-implantation development. M. Metzler M. Metzler Biomedical Research Centre, University of British Columbia, Vancouver, Canada. Search for more papers by this author A. Gertz A. Gertz Biomedical Research Centre, University of British Columbia, Vancouver, Canada. Search for more papers by this author M. Sarkar M. Sarkar Biomedical Research Centre, University of British Columbia, Vancouver, Canada. Search for more papers by this author H. Schachter H. Schachter Biomedical Research Centre, University of British Columbia, Vancouver, Canada. Search for more papers by this author J.W. Schrader J.W. Schrader Biomedical Research Centre, University of British Columbia, Vancouver, Canada. Search for more papers by this author J.D. Marth J.D. Marth Biomedical Research Centre, University of British Columbia, Vancouver, Canada. Search for more papers by this author M. Metzler M. Metzler Biomedical Research Centre, University of British Columbia, Vancouver, Canada. Search for more papers by this author A. Gertz A. Gertz Biomedical Research Centre, University of British Columbia, Vancouver, Canada. Search for more papers by this author M. Sarkar M. Sarkar Biomedical Research Centre, University of British Columbia, Vancouver, Canada. Search for more papers by this author H. Schachter H. Schachter Biomedical Research Centre, University of British Columbia, Vancouver, Canada. Search for more papers by this author J.W. Schrader J.W. Schrader Biomedical Research Centre, University of British Columbia, Vancouver, Canada. Search for more papers by this author J.D. Marth J.D. Marth Biomedical Research Centre, University of British Columbia, Vancouver, Canada. Search for more papers by this author Author Information M. Metzler1, A. Gertz1, M. Sarkar1, H. Schachter1, J.W. Schrader1 and J.D. Marth1 1Biomedical Research Centre, University of British Columbia, Vancouver, Canada. The EMBO Journal (1994)13:2056-2065https://doi.org/10.1002/j.1460-2075.1994.tb06480.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Complex asparagine (N)-linked oligosaccharides appear late in phylogeny and are highly regulated in vertebrates. Variations in these structures are found on the majority of cell-surface and secreted proteins. Complex N-linked oligosaccharide biosynthesis is initiated in the Golgi apparatus by the action of Mgat-1-encoded UDP-N-acetylglucosamine:alpha-3-D- mannoside beta-1,2-N-acetylglucosaminyltransferase I (GlcNAc-TI). To determine if these structures govern ontogenic processes in mammals, mouse embryos were generated that lacked a functional Mgat-1 gene. Inactivation of both Mgat-1 alleles produced deficiencies in GlcNAc-TI activity and complex N-linked oligosaccharides. Embryonic lethality occurred by day 10.5, thus establishing that complex N-linked oligosaccharides are required during post-implantation development. Remarkably, embryonic development proceeded into day 9 with the differentiation of multiple cell types. Complex N-linked oligosaccharides are important for morphogenic processes as neural tube formation, vascularization and the determination of left-right body plan asymmetry were impaired in the absence of a functional Mgat-1 gene. Previous ArticleNext Article Volume 13Issue 91 May 1994In this issue RelatedDetailsLoading ...