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l -Arabinose Isomerase Formation in a Conditional Mutant of Gene araA of Escherichia coli B/r

1972; American Society for Microbiology; Volume: 112; Issue: 3 Linguagem: Inglês

10.1128/jb.112.3.1247-1253.1972

1098-5530

Judith Pauley, Joseph Power, Joseph Irr,

Liver Disease Diagnosis and Treatment

A temperature-sensitive mutant of Escherichia coli in which the synthesis of l -arabinose isomerase is blocked during growth at 42 C was found to possess the following properties. (i) The mutation occurred in the structural gene for the isomerase, gene araA . (ii) During growth at elevated temperatures the mutant accumulates a product which is a precursor to the active enzyme. (iii) The precursor produced at 42 C is slowly converted to active enzyme at 28 C in the absence of protein and ribonucleic acid synthesis. It is concluded that the mutation results in a change in the structure of isomerase which causes formation of active enzyme to be thermolabile at a step beyond the level of translation.