Hybrid type glycolipids (lacto-ganglio series) with a novel branched structure. Their presence in undifferentiated murine leukemia cells and their dependence on differentiation.
1984; Elsevier BV; Volume: 259; Issue: 13 Linguagem: Inglês
10.1016/s0021-9258(17)39751-x
ISSN1083-351X
AutoresReiji Kannagi, Steven B. Levery, Sen‐itiroh Hakomori,
Tópico(s)Chemical Synthesis and Analysis
ResumoGlycosphingolipidshaving a unique branched structure at the 11-galactose residue of lactosylceramide have been isolated and characterized from a cultured murine leukemia cell line in an undifferentiated state (Ml-cells).The structures of the glycosphingolipids were characterized (as shown below) by methylation analysis, direct probe mass spectrometry, proton NMR spectrometry, and degradation with exo-glycosidases.MI-XGL-1 (LC(&,) GalNAcBl 1 4Ga491-4Glcf1141Cer 3 7 GlcNAcOl MI-xGL-3 (V'uGalLcGgd GalNAcBl 1 ~Gal,91+4GlcBl+lCer 7 G d a l + 3 G a l j 3 1 4 G l ~N A ~j 3 1 The structures of these glycolipids are hybrids of the lacto and ganglio series, which are characterized by the presence of GlcNAcB1+3 and GalNAc@1+4 linked to the Gal residue of Gal/31+4Glc~l+lCer.This new hybrid series can be designated as "lacto-ganglio series.*These glycolipids are present in undifferentiated murine leukemia cells.Their concentration declines with differentiation and they are virtually absent in differentiated M1' cells, suggesting that lacto-ganglio structures could be markers of undifferentiated, malignant myeloid cells.
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