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Continuous-Dilution Method for the Mass Culture of Synchronized Cells

1968; American Society for Microbiology; Volume: 16; Issue: 3 Linguagem: Inglês

10.1128/am.16.3.496-499.1968

0003-6919

T.A. Hare, Robert R. Schmidt,

Algal biology and biofuel production

A new mass-culturing technique for synchronized cells is described. Continuous dilution with fresh culture medium provides the cells with nearly constant environmental conditions (continuous resupply of nutrients, constant adjustment of p H, maintenance of a nearly constant cell mass-to-culture volume ratio, etc.) at high cell densities, thereby resulting in the yield of ample cellular material for most biochemical studies during the cell cycle. The new mass-culturing system, when utilized to culture synchronous Chlorella pyrenoidosa , yielded approximately three times as much cellular material as a previous nondilution method, in the same time period and in less than one-tenth the culture volume. Although the newly developed mass-culture method was tested with a unicellular green alga, the basic method should be readily adaptable to synchronous cultures of other microorganisms and to cells in tissue culture.