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Nucleotide sequence of the rodA gene, responsible for the rod shape of Escherichia coli: rodA and the pbpA gene, encoding penicillin-binding protein 2, constitute the rodA operon

1989; American Society for Microbiology; Volume: 171; Issue: 1 Linguagem: Inglês

10.1128/jb.171.1.558-560.1989

1098-5530

Hiroshi Matsuzawa, Sadamitsu Asoh, K Kunai, Kanae Muraiso, Akiko Takasuga, Takehiro Ohta,

Microbial Natural Products and Biosynthesis

The rodA gene, which is responsible for the rod shape of Escherichia coli, was located 5 nucleotides downstream of another rod-shape-determining gene, pbpA, encoding penicillin-binding protein 2. The coding region for the RodA protein was 1,110 base pairs in length. Two plasmids, carrying a rodA-lacZ gene fusion with and without the pbpA promoter upstream of the gene fusion, were constructed. On the basis of the difference between the expression levels of the beta-galactosidase activity dependent on and independent of the pbpA promoter, we concluded that the pbpA and rodA genes constitute a single transcriptional unit called the rodA operon.