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PCR duplex para diferenciação de amostras vacinais e selvagens do vírus da doença de Aujeszky

2010; UNIVERSIDADE FEDERAL DE MINAS GERAIS; Volume: 62; Issue: 5 Linguagem: Inglês

10.1590/s0102-09352010000500032

1678-4162

Antônio Augusto Fonseca Júnior, Natanael Lamas Dias, R.C. Leite, Marcos Bryan Heinemann, Jenner Karlisson Pimenta dos Reis,

Animal Disease Management and Epidemiology

ABSTRACT A duplex PCR was developed to differentiate the wild-type virus from the attenuated virus used in vaccinations. The PCR was able to amplify fragments of 493bp for glycoprotein E (gE) gene and 207bp for glycoprotein B (gB) gene. The analytical sensitivity was determined by addition of a virus field sample titled in the brain samples of pigs. The standard virus strain Shope, the vaccine strain Bartha, and ten other field isolates were subjected to PCR. The PCR was able to amplify fragments of gE and gB in all field samples and only fragments of gB were amplified in the attenuated virus, as expected. The technique was able to detect up to 10 0.5 TCID50/50µL virus in samples of brain. Duplex PCR proved to be an important tool for differentiation of naturally-infected animals and animals vaccinated with the virus deleted for gE . Keywords: Aujeszky´s disease, PCR, vaccine REFERENCIAS BIBLIOGRAFICAS BASCUNANA, C.R.; BJORNEROT, L.; BALLAGI-PORDANY, A. et al. Detection of pseudorabies virus genomic sequences in apparently uninfected “single reactor” pigs.