Multiplex-Polymerase Chain Reaction Assay for the Authentication of the Mackerel Scomber colias in Commercial Canned Products
2006; Oxford University Press; Volume: 89; Issue: 3 Linguagem: Inglês
10.1093/jaoac/89.3.708
ISSN1944-7922
AutoresCarlos Infante, Manuel Manchado,
Tópico(s)Meat and Animal Product Quality
ResumoA multiplex-polymerase chain reaction (PCR) system was developed for the authentication of the mackerel Scomber colias in commercial canned products. This novel method consists of an S. colias-specific fragment [159 base pairs (bp)] located in the nontranscribed spacer (NTS) sequence, and a Scomber genus-specific PCR product in the 5S rRNA gene (196-201 bp) as a positive amplification control. The system was assayed using 18 different canned products labeled as S. colias. A positive identification was made in all but one sample, revealing this methodology as a potential molecular tool for direct application in the authentication of S. colias canned products.
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