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Ribonucleic Acid Code Words in Wheat Germ

1966; Elsevier BV; Volume: 241; Issue: 8 Linguagem: Inglês

10.1016/s0021-9258(18)96724-4

1083-351X

Carlos Basilio, María Cecilia Bravo, Jorge E. Allende,

Advanced biosensing and bioanalysis techniques

An amino acid-incorporating system has been obtained from ungerminated wheat germ, which can be stimulated by the addition of uridylic-rich polynucleotides. Polyadenylic and polycytidylic acids fail to stimulate the incorporation of lysine and proline, respectively. The amino acid codons determined by the use of the uridylic-rich polynucleotides coincide almost perfectly with those reported for Escherichia coli. Binding of aminoacyl-sRNA to wheat germ ribosomes can be directed specifically by polynucleotides and does not require the presence of supernatant fraction or guanosine triphosphate. Polycytidylic acid failed to promote prolyl-sRNA binding by ribosomes.