Rice gene expression profiles responding to larval feeding of the striped stem borer at the 1st to 2nd instar stage
2010; Wiley; Volume: 18; Issue: 3 Linguagem: Inglês
10.1111/j.1744-7917.2010.01372.x
ISSN1744-7917
AutoresYang Sun, Yongjun Zhang, Guangchun Cao, Shaohua Gu, Kongming Wu, Xiwu Gao, Yuyuan Guo,
Tópico(s)Entomopathogenic Microorganisms in Pest Control
ResumoInsect ScienceVolume 18, Issue 3 p. 273-281 ORIGINAL ARTICLE Rice gene expression profiles responding to larval feeding of the striped stem borer at the 1st to 2nd instar stage Yang Sun, Yang Sun State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing College of Agriculture and Biotechnology, China Agricultural University, Beijing, ChinaSearch for more papers by this authorYong-Jun Zhang, Yong-Jun Zhang State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, BeijingSearch for more papers by this authorGuang-Chun Cao, Guang-Chun Cao State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, BeijingSearch for more papers by this authorShao-Hua Gu, Shao-Hua Gu State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, BeijingSearch for more papers by this authorKong-Ming Wu, Kong-Ming Wu State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, BeijingSearch for more papers by this authorXi-Wu Gao, Xi-Wu Gao College of Agriculture and Biotechnology, China Agricultural University, Beijing, ChinaSearch for more papers by this authorYu-Yuan Guo, Yu-Yuan Guo State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, BeijingSearch for more papers by this author Yang Sun, Yang Sun State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing College of Agriculture and Biotechnology, China Agricultural University, Beijing, ChinaSearch for more papers by this authorYong-Jun Zhang, Yong-Jun Zhang State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, BeijingSearch for more papers by this authorGuang-Chun Cao, Guang-Chun Cao State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, BeijingSearch for more papers by this authorShao-Hua Gu, Shao-Hua Gu State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, BeijingSearch for more papers by this authorKong-Ming Wu, Kong-Ming Wu State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, BeijingSearch for more papers by this authorXi-Wu Gao, Xi-Wu Gao College of Agriculture and Biotechnology, China Agricultural University, Beijing, ChinaSearch for more papers by this authorYu-Yuan Guo, Yu-Yuan Guo State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, BeijingSearch for more papers by this author First published: 20 December 2010 https://doi.org/10.1111/j.1744-7917.2010.01372.xCitations: 6 Yu-Yuan Guo, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Yuanmingyuan West Rd. 2, 100193, Beijing, China. Email: yuyuanguo@hotmail.com Note: The first two authors contributed equally to this paper. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Abstract The objective of this study was to identify rice genes that are in response to the striped stem borer (SSB) (Chilo suppressalis Walker) feeding at the first to second larval stage. Using combined suppression subtractive hybridization (SSH) and dot blot approaches, we analyzed the induced defense genes that took place during the first 72 h of infesting intact rice (Oryza sativa L.) plants in sheath tissues with SSB larvae. By sequencing the whole SSH library, 39 expressed sequence tags involved in disease stress, insect stress or other stress responses were identified to be up-regulated by SSB larvae feeding. Among these genes, rice allene oxide cyclase (AOC), terpene synthase (TPS) and four proteinase inhibitor (PI) genes were up-regulated by SSB larvae feeding. Real-time quantitative reverse transcription polymerase chain reaction analysis showed that four rice PI genes were already up-regulated at 6 h, and reached peaks between 6 h to 12 h. In addition, the transcription of gene involving in jasmonate signaling pathway such as allene oxide cyclase (AOC) concerning rice early defense response to SSB feeding was activated after rice feeding by SSB for 2 h. Although the expression of rice terpene synthase (TPS) gene, involved in the biosynthesis of monoterpenes or diterpenes, was already up-regulated at 7 h, a significant increase in the expression was delayed until 12 h and reached its peak at 24 h. The present study identified six SSB-response genes and their expression patterns, which provides evidence and information to understand insect stress-response in plants. Citing Literature Supporting Information Table S1 Oligonucleotide primers sets for real-time polymerase chain reaction. Table S2 Amplification efficiencies (±SD) of the four rice proteinase inhibitor genes and rice Allene oxide cyclase genes and reference gene measured by 12 series of treatment times and average Ct values (±SD) obtained by measuring expression in the biological replicates of these rice genes. Filename Description INS_1372_sm_TableS1-S2.doc95.5 KB Supporting info item Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume18, Issue3June 2011Pages 273-281 RelatedInformation
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