Paper Chromatography of Carbohydrates and Related Compounds
1954; Academic Press; Linguagem: Inglês
10.1016/s0096-5332(08)60379-3
ISSN1875-6425
Autores Tópico(s)Biochemical Analysis and Sensing Techniques
ResumoThis chapter provides an overview of paper chromatography of carbohydrates and related compounds. The chapter surveys the application of filter-paper chromatography to carbohydrates and certain of their derivatives. It discusses the different methods of arranging the filter paper in the chromatographic chamber— descending, ascending, and circular or horizontal development. The filter paper most commonly used is Whatman No. 1. Many different solvent developers are used in the separation of sugars and related compounds. Three of these, phenol–water, collidine–water, and l-butanol–acetic acid–water, also commonly employed in the resolution of amino acid mixtures, are widely used. On theoretical grounds, low retention factor (RF) values, in the range of 0.2 to 0.3, are most desirable for maximum separations. The size of the areas occupied by the spots results in overlapping at low RF values, even though the partition coefficients are favorable for separation.
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