Особенности введения в культуру in vitro боярышника перстонадрезанного (Crataegus pinnatifida Bunge)
2014; Linguagem: Inglês
ISSN
1996-4277
AutoresФирсова Мария Владимировна, Набиева Александра Юрьевна,
Tópico(s)Fungal Biology and Applications
ResumoThe wide use of С. pinnatifida in landscaping is restricted because of the difficulties of its cloning by traditional methods. The goal of our investigation was the study of morphogenetic potential of C. pinnatifida isolated buds and the peculiarities of their tissue culture initiation. The following objectives were involved: to reveal the optimal time for buds initiation in tissue culture, to fit sterilization agent and the best culture medium for active shoot proliferation; to study buds morphogenetic reaction due to their position on the stem and growth regulators added. Explants were taken twice: from February to April and from September to November. It was found that buds isolated into tissue culture in March-April had the best morphogenetic potential. Three different agents were used for buds sterilization. The best effect of sterilization (85% viable buds) was obtained when 0.1% HgCl2 solution was used. Five different nutrient media were used: МS, WPM, Kn, DKW, Nas and Read with agar and sucrose. The highest axillary shoot multiplication was obtained on Nas and Read medium when 6benzylaminopurine and kinetin in concentration of 0.25-0.5 mg L were added simultaneously. The elongation of isolated microshoots was obtained by the use of 0.5 mg L GA3 added to the regeneration medium. The majority of apical buds of C. pinnatifida gave microshoots with flowers in vitro. After adding to MS medium 0.5 mg L IAA, 0.2 mg L 2.4-D, 0.5 mg L kinetin and 0.5 mg L 6-benzylaminopurine, the frequency of morphogenic callus induction reached 81.0-86.0%. For rooting of the obtained microshoots we used Knudson medium or Ѕ MS medium with 0.5 mg L IBA added. The peculiarities of clonal micropropagation of C. pinnatifida using axillary bud explants were revealed and the role of biotic and abiotic factors influencing shoot morphogenesis was analyzed.
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