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Comparison of four charcoal media for the isolation of Bordetella pertussis

1989; American Society for Microbiology; Volume: 27; Issue: 5 Linguagem: Inglês

10.1128/jcm.27.5.1097-1098.1989

1098-660X

J. E. Hoppe, J. Schwaderer,

Microbial infections and disease research

Charcoal-horse blood agar with 40 micrograms of cephalexin per ml, charcoal-horse blood agar with 3 micrograms of lincomycin per ml, charcoal agar with 3 micrograms of lincomycin per ml, and Legionella (buffered charcoal-yeast extract) agar with 3 micrograms of lincomycin per ml were compared for isolation of Bordetella pertussis. Charcoal-horse blood agar with 40 micrograms of cephalexin per ml gave the best results, with a B. pertussis recovery rate of 100%. Growth was most rapid and the mean number of colonies was highest on this agar, and growth of pharyngeal flora was completely suppressed.