Stem-Loop RT-qPCR for MicroRNA Expression Profiling
2011; Springer Science+Business Media; Linguagem: Inglês
10.1007/978-1-61779-427-8_3
ISSN1940-6029
AutoresJames Hurley, Doug N Roberts, Andrew T. Bond, David N. Keys, Caifu Chen,
Tópico(s)Cancer-related molecular mechanisms research
ResumoQuantification of the microRNAs (miRNAs) in cells or tissues is a crucial step in understanding their biological functions. Development of the stem-loop reverse transcription procedure and TaqMan® miRNA assays enables accurate detection of miRNA expression levels by quantitative PCR. Increased experimental throughput permits the expression screening of larger number of miRNAs with small amounts of sample. Here, we demonstrate the use of both TaqMan® Array Card and OpenArray® platforms to accurately determine the level of miRNA gene expression in biological samples.
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